{"id":4231,"date":"2015-10-09T12:32:09","date_gmt":"2015-10-09T10:32:09","guid":{"rendered":"http:\/\/botanico.uclm.es\/project\/genetic-characterization-and-variation-within-and-among-populations-of-anthyllis-rupestris-coss-and-endangered-endemism-of-southern-spain-2\/"},"modified":"2022-12-21T18:31:31","modified_gmt":"2022-12-21T17:31:31","slug":"genetic-characterization-and-variation-within-and-among-populations-of-anthyllis-rupestris-coss-and-endangered-endemism-of-southern-spain","status":"publish","type":"project","link":"https:\/\/botanico.uclm.es\/en\/project\/genetic-characterization-and-variation-within-and-among-populations-of-anthyllis-rupestris-coss-and-endangered-endemism-of-southern-spain\/","title":{"rendered":"Genetic characterization and variation within and among populations of Anthyllis rupestris Coss. and endangered endemism of southern Spain"},"content":{"rendered":"<style type=\"text\/css\"> \n#cmsms_row_69e91d2c0299d .cmsms_row_outer_parent { \n\tpadding-top: 0px; \n} \n\n#cmsms_row_69e91d2c0299d .cmsms_row_outer_parent { \n\tpadding-bottom: 50px; \n} \n\n\n#cmsms_button_69e91d2c0a7d2 { \n\ttext-align:center; \n} \n\n#cmsms_button_69e91d2c0a7d2 .cmsms_button:before { \n\tmargin-right:.5em; \n\tmargin-left:0; \n\tvertical-align:baseline; \n} \n\n#cmsms_button_69e91d2c0a7d2 .cmsms_button { \n\tfont-weight:100; \n\tfont-style:normal; \n\tpadding-right:20px; \n\tpadding-left:20px; \n} \n#cmsms_button_69e91d2c0a7d2 .cmsms_button:hover { \n} \n<\/style><div id=\"cmsms_row_69e91d2c0299d\" class=\"cmsms_row cmsms_color_scheme_default\">\n<div class=\"cmsms_row_outer_parent\">\n<div class=\"cmsms_row_outer\">\n<div class=\"cmsms_row_inner\">\n<div class=\"cmsms_row_margin\">\n<div class=\"cmsms_column one_first\">\n<div class=\"cmsms_text\">\n<h2 id=\"author1\" class=\"secHeading\">Abstract<\/h2>\n<p id=\"abspara0010\"><em>Anthillys rupestris<\/em> Coss. is a rare, endangered and endemic plant from the south of the Iberian Peninsula. The phylogenetic relationships of <em>A. rupestris<\/em> and its genetic variation were studied to assess the distribution of genetic variability within and among six subpopulations from two remnants locations. We obtained sequences of internal transcribed spacer ITS2 of three <em>Anthyllis<\/em> species and ISSRs markers to estimate gene diversity and the genetic structure of the subpopulations. <em>A. rupestris<\/em> was closely related to two other endemic species to southeastern Spain. The ISSR analyses revealed high level of genetic diversity [61.59% average polymorphism; and <em>H<\/em><sub>T<\/sub>\u00a0=\u00a00.269] in subpopulations and a moderate level of genetic differentiation [(<em>G<\/em><sub>ST<\/sub>)\u00a0=\u00a00.267] among subpopulations. Furthermore, analysis of molecular variance (AMOVA) revealed that 18% of the total genetic diversity resided among the two analysed areas, 12% among subpopulations and 70% variation was found within subpopulations. Principal Coordinates Analysis (PCA) partially grouped together the genotypes according to their geographical origin. Three out of the four subpopulations from one location were genetically related to each other, and the remaining subpopulation, which was fenced, was isolated from the obtained clusters. The data are interpreted as a result of recent fragmentation and isolation. Threats to this species are mainly anthropogenic. Because\u00a0<em>A. rupestris<\/em> currently retains a high genetic variation among individuals within subpopulations, a metapopulation perspective preserving and expanding the habitat at each site, in order to promote connectivity would be an additional strategy to ensure conservation before populations become too small to persist naturally.<\/p>\n<p id=\"kwd_1\" class=\"svKeywords\"><strong>Keywords:\u00a0<\/strong><span id=\"\"><em>Anthyllis, <\/em><\/span><span id=\"\">Endemic,\u00a0<\/span><span id=\"\">Habitat fragmentation,\u00a0<\/span><span id=\"\">ISSR,\u00a0<\/span><span id=\"\">ITS,\u00a0<\/span>Population genetic structure.<\/p>\n<\/div>\n\n<div id=\"cmsms_button_69e91d2c0a7d2\" class=\"button_wrap\"><a href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S030519781200155X\" class=\"cmsms_button\" target=\"_blank\">Link to the article<\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":4229,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","format":"standard","meta":[],"pj-categs":[79],"pj-tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v19.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Genetic characterization and variation within and among populations of Anthyllis rupestris Coss. and endangered endemism of southern Spain - Instituto Bot\u00e1nico<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/botanico.uclm.es\/en\/project\/genetic-characterization-and-variation-within-and-among-populations-of-anthyllis-rupestris-coss-and-endangered-endemism-of-southern-spain\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Genetic characterization and variation within and among populations of Anthyllis rupestris Coss. and endangered endemism of southern Spain - Instituto Bot\u00e1nico\" \/>\n<meta property=\"og:url\" content=\"https:\/\/botanico.uclm.es\/en\/project\/genetic-characterization-and-variation-within-and-among-populations-of-anthyllis-rupestris-coss-and-endangered-endemism-of-southern-spain\/\" \/>\n<meta property=\"og:site_name\" content=\"Instituto Bot\u00e1nico\" \/>\n<meta property=\"article:modified_time\" content=\"2022-12-21T17:31:31+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/botanico.uclm.es\/wp-content\/uploads\/2015\/10\/Captura-de-pantalla-2015-10-09-a-las-12.31.23.png\" \/>\n\t<meta property=\"og:image:width\" content=\"728\" \/>\n\t<meta property=\"og:image:height\" content=\"854\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/botanico.uclm.es\/en\/project\/genetic-characterization-and-variation-within-and-among-populations-of-anthyllis-rupestris-coss-and-endangered-endemism-of-southern-spain\/\",\"url\":\"https:\/\/botanico.uclm.es\/en\/project\/genetic-characterization-and-variation-within-and-among-populations-of-anthyllis-rupestris-coss-and-endangered-endemism-of-southern-spain\/\",\"name\":\"Genetic characterization and variation within and among populations of Anthyllis rupestris Coss. and endangered endemism of southern Spain - 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