{"id":4415,"date":"2015-11-13T10:23:32","date_gmt":"2015-11-13T09:23:32","guid":{"rendered":"http:\/\/botanico.uclm.es\/en\/project\/genomic-organization-of-a-udp-glucosyltransferase-gene-determines-differential-accumulation-of-specific-flavonoid-glucosides-in-tepals\/"},"modified":"2022-12-22T17:27:11","modified_gmt":"2022-12-22T16:27:11","slug":"genomic-organization-of-a-udp-glucosyltransferase-gene-determines-differential-accumulation-of-specific-flavonoid-glucosides-in-tepals","status":"publish","type":"project","link":"https:\/\/botanico.uclm.es\/en\/project\/genomic-organization-of-a-udp-glucosyltransferase-gene-determines-differential-accumulation-of-specific-flavonoid-glucosides-in-tepals\/","title":{"rendered":"Genomic organization of a UDP-glucosyltransferase gene determines differential accumulation of specific flavonoid glucosides in tepals"},"content":{"rendered":"<style type=\"text\/css\"> \n#cmsms_row_69e5cbba6a543 .cmsms_row_outer_parent { \n\tpadding-top: 0px; \n} \n\n#cmsms_row_69e5cbba6a543 .cmsms_row_outer_parent { \n\tpadding-bottom: 50px; \n} \n\n\n#cmsms_button_69e5cbba6a707 { \n\ttext-align:center; \n} \n\n#cmsms_button_69e5cbba6a707 .cmsms_button:before { \n\tmargin-right:.5em; \n\tmargin-left:0; \n\tvertical-align:baseline; \n} \n\n#cmsms_button_69e5cbba6a707 .cmsms_button { \n\tfont-weight:100; \n\tfont-style:normal; \n\tpadding-right:20px; \n\tpadding-left:20px; \n} \n#cmsms_button_69e5cbba6a707 .cmsms_button:hover { \n} \n<\/style><div id=\"cmsms_row_69e5cbba6a543\" class=\"cmsms_row cmsms_color_scheme_default\">\n<div class=\"cmsms_row_outer_parent\">\n<div class=\"cmsms_row_outer\">\n<div class=\"cmsms_row_inner\">\n<div class=\"cmsms_row_margin\">\n<div class=\"cmsms_column one_first\">\n<div class=\"cmsms_text\">\n<h2 class=\"Heading\">Abstract<\/h2>\n<p>Glycosylation plays a major role in the chemical diversity of flavonoids. The wide diversity of the family-1 glycosyltransferase (UGT) impairs the determination of the biochemical function solely from its primary sequence. Here we combined differential expression and target metabolomic analysis in various Crocus species to identify a gene that is key in determining the flavonoid composition of Crocus species that belong to the Crocus series. UGT703B1 recognizes isorhamnetin and kaempferol as substrates in vitro. In addition, UGT703B1 expression was found to be highly correlated with the presence of kaempferol 7-O-biglucoside-3-O-\u03b2-glucoside and isorhamnetin-3,7-O-diglucoside. These flavonols were present in C. sativus and C. cartwrightianusalbus, both from series Crocus but absent in Crocus species from the other series analyzed. Further, the presence of both flavonols was associated with the expression of UGT703B1, and this expression was correlated with the presence of the UGT703B1 coding gene, with the exception of C. cancellatus, whose genomic sequence was present but contained a shorter intronic sequence and promoter alterations, suggesting the presence of regulatory sequences in the deleted part of that intron and promoter important for UGT703B1 expression. Overall, the data obtained supports the involvement of UGT703B1 in the formation of specific kaempferol and isorhamnetin glucosides, while demonstrating that the integration of metabolomic and differential expression analysis is a versatile tool for understanding a multigene family of UGTs in Crocus. \u00a9 2014, Springer Science+Business Media Dordrecht.<\/p>\n<p><strong>Keywords:<\/strong> Crocus, Flavonoids, Glucosyltransferase, Intron, Promoter, Tepals<\/p>\n<\/div>\n\n<div id=\"cmsms_button_69e5cbba6a707\" class=\"button_wrap\"><a href=\"http:\/\/www.scopus.com\/inward\/record.url?eid=2-s2.0-84910113077&#038;partnerID=40&#038;md5=948f8111578dd321aa2ed4de141f4b34\" class=\"cmsms_button\" target=\"_blank\">Link to the article<\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":4355,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","format":"standard","meta":[],"pj-categs":[74],"pj-tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v19.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Genomic organization of a UDP-glucosyltransferase gene determines differential accumulation of specific flavonoid glucosides in tepals - Instituto Bot\u00e1nico<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/botanico.uclm.es\/en\/project\/genomic-organization-of-a-udp-glucosyltransferase-gene-determines-differential-accumulation-of-specific-flavonoid-glucosides-in-tepals\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Genomic organization of a UDP-glucosyltransferase gene determines differential accumulation of specific flavonoid glucosides in tepals - Instituto Bot\u00e1nico\" \/>\n<meta property=\"og:url\" content=\"https:\/\/botanico.uclm.es\/en\/project\/genomic-organization-of-a-udp-glucosyltransferase-gene-determines-differential-accumulation-of-specific-flavonoid-glucosides-in-tepals\/\" \/>\n<meta property=\"og:site_name\" content=\"Instituto Bot\u00e1nico\" \/>\n<meta property=\"article:modified_time\" content=\"2022-12-22T16:27:11+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/botanico.uclm.es\/wp-content\/uploads\/2015\/09\/IMG_39641.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1920\" \/>\n\t<meta property=\"og:image:height\" content=\"1440\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/botanico.uclm.es\/en\/project\/genomic-organization-of-a-udp-glucosyltransferase-gene-determines-differential-accumulation-of-specific-flavonoid-glucosides-in-tepals\/\",\"url\":\"https:\/\/botanico.uclm.es\/en\/project\/genomic-organization-of-a-udp-glucosyltransferase-gene-determines-differential-accumulation-of-specific-flavonoid-glucosides-in-tepals\/\",\"name\":\"Genomic organization of a UDP-glucosyltransferase gene determines differential accumulation of specific flavonoid glucosides in tepals - 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Instituto Bot\u00e1nico","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/botanico.uclm.es\/en\/project\/genomic-organization-of-a-udp-glucosyltransferase-gene-determines-differential-accumulation-of-specific-flavonoid-glucosides-in-tepals\/","og_locale":"en_US","og_type":"article","og_title":"Genomic organization of a UDP-glucosyltransferase gene determines differential accumulation of specific flavonoid glucosides in tepals - Instituto Bot\u00e1nico","og_url":"https:\/\/botanico.uclm.es\/en\/project\/genomic-organization-of-a-udp-glucosyltransferase-gene-determines-differential-accumulation-of-specific-flavonoid-glucosides-in-tepals\/","og_site_name":"Instituto Bot\u00e1nico","article_modified_time":"2022-12-22T16:27:11+00:00","og_image":[{"width":1920,"height":1440,"url":"https:\/\/botanico.uclm.es\/wp-content\/uploads\/2015\/09\/IMG_39641.jpg","type":"image\/jpeg"}],"twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/botanico.uclm.es\/en\/project\/genomic-organization-of-a-udp-glucosyltransferase-gene-determines-differential-accumulation-of-specific-flavonoid-glucosides-in-tepals\/","url":"https:\/\/botanico.uclm.es\/en\/project\/genomic-organization-of-a-udp-glucosyltransferase-gene-determines-differential-accumulation-of-specific-flavonoid-glucosides-in-tepals\/","name":"Genomic organization of a UDP-glucosyltransferase gene determines differential accumulation of specific flavonoid glucosides in tepals - 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