{"id":6490,"date":"2021-12-19T10:06:03","date_gmt":"2021-12-19T09:06:03","guid":{"rendered":"https:\/\/botanico.uclm.es\/en\/project\/identification-and-characterization-of-apocarotenoid-modifiers-and-carotenogenic-enzymes-for-biosynthesis-of-crocins-in-buddleja-davidii-flowers\/"},"modified":"2022-12-21T17:18:56","modified_gmt":"2022-12-21T16:18:56","slug":"identification-and-characterization-of-apocarotenoid-modifiers-and-carotenogenic-enzymes-for-biosynthesis-of-crocins-in-buddleja-davidii-flowers","status":"publish","type":"project","link":"https:\/\/botanico.uclm.es\/en\/project\/identification-and-characterization-of-apocarotenoid-modifiers-and-carotenogenic-enzymes-for-biosynthesis-of-crocins-in-buddleja-davidii-flowers\/","title":{"rendered":"Identification and characterization of apocarotenoid modifiers and carotenogenic enzymes for biosynthesis of crocins in Buddleja davidii flowers"},"content":{"rendered":"<style type=\"text\/css\"> \n#cmsms_row_69e9d6c6bc1c5 .cmsms_row_outer_parent { \n\tpadding-top: 0px; \n} \n\n#cmsms_row_69e9d6c6bc1c5 .cmsms_row_outer_parent { \n\tpadding-bottom: 50px; \n} \n\n<\/style><div id=\"cmsms_row_69e9d6c6bc1c5\" class=\"cmsms_row cmsms_color_scheme_default\">\n<div class=\"cmsms_row_outer_parent\">\n<div class=\"cmsms_row_outer\">\n<div class=\"cmsms_row_inner\">\n<div class=\"cmsms_row_margin\">\n<div class=\"cmsms_column one_first\">\n<div class=\"cmsms_text\">\n<h2 class=\"Heading\">Resumen<\/h2>\n<p>Crocetin biosynthesis in Buddleja davidii flowers proceeds through a zeaxanthin cleavage pathway catalyzed by two carotenoid cleavage dioxygenases (BdCCD4.1 and BdCCD4.3), followed by oxidation and glucosylation reactions that lead to the production of crocins. We isolated and analyzed the expression of 12 genes from the carotenoid pathway in B. davidii flowers and identified four candidate genes involved in the biosynthesis of crocins (BdALDH, BdUGT74BC1, BdUGT74BC2, and BdUGT94AA3). In addition, we characterized the profile of crocins and their carotenoid precursors, following their accumulation during flower development. Overall, seven different crocins, crocetin, and picrocrocin were identified in this study. The accumulation of these apocarotenoids parallels tissue development, reaching the highest concentration when the flower is fully open. Notably, the pathway was regulated mainly at the transcript level, with expression patterns of a large group of carotenoid precursor and apocarotenoid genes (BdPSY2, BdPDS2, BdZDS, BdLCY2, BdBCH, BdALDH, and BdUGT Genes) mimicking the accumulation of crocins. Finally, we used comparative correlation network analysis to study how the synthesis of these valuable apocarotenoids diverges among B. davidii, Gardenia jasminoides, and Crocus sativus, highlighting distinctive differences which could be the basis of the differential accumulation of crocins in the three species. \u00a9 2021 The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","format":"standard","meta":[],"pj-categs":[74],"pj-tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v19.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Identification and characterization of apocarotenoid modifiers and carotenogenic enzymes for biosynthesis of crocins in Buddleja davidii flowers - Instituto Bot\u00e1nico<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/botanico.uclm.es\/en\/project\/identification-and-characterization-of-apocarotenoid-modifiers-and-carotenogenic-enzymes-for-biosynthesis-of-crocins-in-buddleja-davidii-flowers\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Identification and characterization of apocarotenoid modifiers and carotenogenic enzymes for biosynthesis of crocins in Buddleja davidii flowers - Instituto Bot\u00e1nico\" \/>\n<meta property=\"og:url\" content=\"https:\/\/botanico.uclm.es\/en\/project\/identification-and-characterization-of-apocarotenoid-modifiers-and-carotenogenic-enzymes-for-biosynthesis-of-crocins-in-buddleja-davidii-flowers\/\" \/>\n<meta property=\"og:site_name\" content=\"Instituto Bot\u00e1nico\" \/>\n<meta property=\"article:modified_time\" content=\"2022-12-21T16:18:56+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/botanico.uclm.es\/en\/project\/identification-and-characterization-of-apocarotenoid-modifiers-and-carotenogenic-enzymes-for-biosynthesis-of-crocins-in-buddleja-davidii-flowers\/\",\"url\":\"https:\/\/botanico.uclm.es\/en\/project\/identification-and-characterization-of-apocarotenoid-modifiers-and-carotenogenic-enzymes-for-biosynthesis-of-crocins-in-buddleja-davidii-flowers\/\",\"name\":\"Identification and characterization of apocarotenoid modifiers and carotenogenic enzymes for biosynthesis of crocins in Buddleja davidii flowers - 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Instituto Bot\u00e1nico","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/botanico.uclm.es\/en\/project\/identification-and-characterization-of-apocarotenoid-modifiers-and-carotenogenic-enzymes-for-biosynthesis-of-crocins-in-buddleja-davidii-flowers\/","og_locale":"en_US","og_type":"article","og_title":"Identification and characterization of apocarotenoid modifiers and carotenogenic enzymes for biosynthesis of crocins in Buddleja davidii flowers - Instituto Bot\u00e1nico","og_url":"https:\/\/botanico.uclm.es\/en\/project\/identification-and-characterization-of-apocarotenoid-modifiers-and-carotenogenic-enzymes-for-biosynthesis-of-crocins-in-buddleja-davidii-flowers\/","og_site_name":"Instituto Bot\u00e1nico","article_modified_time":"2022-12-21T16:18:56+00:00","twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/botanico.uclm.es\/en\/project\/identification-and-characterization-of-apocarotenoid-modifiers-and-carotenogenic-enzymes-for-biosynthesis-of-crocins-in-buddleja-davidii-flowers\/","url":"https:\/\/botanico.uclm.es\/en\/project\/identification-and-characterization-of-apocarotenoid-modifiers-and-carotenogenic-enzymes-for-biosynthesis-of-crocins-in-buddleja-davidii-flowers\/","name":"Identification and characterization of apocarotenoid modifiers and carotenogenic enzymes for biosynthesis of crocins in Buddleja davidii flowers - 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