{"id":6522,"date":"2020-12-19T10:15:31","date_gmt":"2020-12-19T09:15:31","guid":{"rendered":"https:\/\/botanico.uclm.es\/en\/project\/efficient-production-of-saffron-crocins-and-picrocrocin-in-nicotiana-benthamiana-using-a-virus-driven-system\/"},"modified":"2022-12-21T17:29:59","modified_gmt":"2022-12-21T16:29:59","slug":"efficient-production-of-saffron-crocins-and-picrocrocin-in-nicotiana-benthamiana-using-a-virus-driven-system","status":"publish","type":"project","link":"https:\/\/botanico.uclm.es\/en\/project\/efficient-production-of-saffron-crocins-and-picrocrocin-in-nicotiana-benthamiana-using-a-virus-driven-system\/","title":{"rendered":"Efficient production of saffron crocins and picrocrocin in Nicotiana benthamiana using a virus-driven system"},"content":{"rendered":"<style type=\"text\/css\"> \n#cmsms_row_69eccec73d7e0 .cmsms_row_outer_parent { \n\tpadding-top: 0px; \n} \n\n#cmsms_row_69eccec73d7e0 .cmsms_row_outer_parent { \n\tpadding-bottom: 50px; \n} \n\n<\/style><div id=\"cmsms_row_69eccec73d7e0\" class=\"cmsms_row cmsms_color_scheme_default\">\n<div class=\"cmsms_row_outer_parent\">\n<div class=\"cmsms_row_outer\">\n<div class=\"cmsms_row_inner\">\n<div class=\"cmsms_row_margin\">\n<div class=\"cmsms_column one_first\">\n<div class=\"cmsms_text\">\n<h2 class=\"Heading\">Resumen<\/h2>\n<p>Crocins and picrocrocin are glycosylated apocarotenoids responsible, respectively, for the color and the unique taste of the saffron spice, known as red gold due to its high price. Several studies have also shown the health-promoting properties of these compounds. However, their high costs hamper the wide use of these metabolites in the pharmaceutical sector. We have developed a virus-driven system to produce remarkable amounts of crocins and picrocrocin in adult Nicotiana benthamiana plants in only two weeks. The system consists of viral clones derived from tobacco etch potyvirus that express specific carotenoid cleavage dioxygenase (CCD) enzymes from Crocus sativus and Buddleja davidii. Metabolic analyses of infected tissues demonstrated that the sole virus-driven expression of C. sativus CsCCD2L or B. davidii BdCCD4.1 resulted in the production of crocins, picrocrocin and safranal. Using the recombinant virus that expressed CsCCD2L, accumulations of 0.2% of crocins and 0.8% of picrocrocin in leaf dry weight were reached in only two weeks. In an attempt to improve apocarotenoid content in N. benthamiana, co-expression of CsCCD2L with other carotenogenic enzymes, such as Pantoea ananatis phytoene synthase (PaCrtB) and saffron \u03b2-carotene hydroxylase 2 (BCH2), was performed using the same viral system. This combinatorial approach led to an additional crocin increase up to 0.35% in leaves in which CsCCD2L and PaCrtB were co-expressed. Considering that saffron apocarotenoids are costly harvested from flower stigma once a year, and that Buddleja spp. flowers accumulate lower amounts, this system may be an attractive alternative for the sustainable production of these appreciated metabolites.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","format":"standard","meta":[],"pj-categs":[74],"pj-tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v19.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Efficient production of saffron crocins and picrocrocin in Nicotiana benthamiana using a virus-driven system - Instituto Bot\u00e1nico<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/botanico.uclm.es\/en\/project\/efficient-production-of-saffron-crocins-and-picrocrocin-in-nicotiana-benthamiana-using-a-virus-driven-system\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Efficient production of saffron crocins and picrocrocin in Nicotiana benthamiana using a virus-driven system - Instituto Bot\u00e1nico\" \/>\n<meta property=\"og:url\" content=\"https:\/\/botanico.uclm.es\/en\/project\/efficient-production-of-saffron-crocins-and-picrocrocin-in-nicotiana-benthamiana-using-a-virus-driven-system\/\" \/>\n<meta property=\"og:site_name\" content=\"Instituto Bot\u00e1nico\" \/>\n<meta property=\"article:modified_time\" content=\"2022-12-21T16:29:59+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/botanico.uclm.es\/en\/project\/efficient-production-of-saffron-crocins-and-picrocrocin-in-nicotiana-benthamiana-using-a-virus-driven-system\/\",\"url\":\"https:\/\/botanico.uclm.es\/en\/project\/efficient-production-of-saffron-crocins-and-picrocrocin-in-nicotiana-benthamiana-using-a-virus-driven-system\/\",\"name\":\"Efficient production of saffron crocins and picrocrocin in Nicotiana benthamiana using a virus-driven system - 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