{"id":7005,"date":"2016-12-28T10:52:56","date_gmt":"2016-12-28T09:52:56","guid":{"rendered":"https:\/\/botanico.uclm.es\/en\/project\/identification-and-cloning-of-differentially-expressed-soul-and-elip-genes-in-saffron-stigmas-using-a-subtractive-hybridization-approach\/"},"modified":"2022-12-22T17:21:18","modified_gmt":"2022-12-22T16:21:18","slug":"identification-and-cloning-of-differentially-expressed-soul-and-elip-genes-in-saffron-stigmas-using-a-subtractive-hybridization-approach","status":"publish","type":"project","link":"https:\/\/botanico.uclm.es\/en\/project\/identification-and-cloning-of-differentially-expressed-soul-and-elip-genes-in-saffron-stigmas-using-a-subtractive-hybridization-approach\/","title":{"rendered":"Identification and Cloning of Differentially Expressed SOUL and ELIP Genes in Saffron Stigmas Using a Subtractive Hybridization Approach."},"content":{"rendered":"<style type=\"text\/css\"> \n#cmsms_row_69eccec74277b .cmsms_row_outer_parent { \n\tpadding-top: 0px; \n} \n\n#cmsms_row_69eccec74277b .cmsms_row_outer_parent { \n\tpadding-bottom: 50px; \n} \n\n<\/style><div id=\"cmsms_row_69eccec74277b\" class=\"cmsms_row cmsms_color_scheme_default\">\n<div class=\"cmsms_row_outer_parent\">\n<div class=\"cmsms_row_outer\">\n<div class=\"cmsms_row_inner\">\n<div class=\"cmsms_row_margin\">\n<div class=\"cmsms_column one_first\">\n<div class=\"cmsms_text\">\n<h2 class=\"Heading\">Resumen<\/h2>\n<p>Using a subtractive hybridization approach, differentially expressed genes involved in the light response in saffron stigmas were identified. Twenty-two differentially expressed transcript-derived fragments were cloned and sequenced. Two of them were highly induced by light and had sequence similarity to early inducible proteins (ELIP) and SOUL heme-binding proteins. Using these sequences, we searched for other family members expressed in saffron stigma. ELIP and SOUL are represented by small gene families in saffron, with four and five members, respectively. The expression of these genes was analyzed during the development of the stigma and in light and dark conditions. ELIP transcripts were detected in all the developmental stages showing much higher expression levels in the developed stigmas of saffron and all were up-regulated by light but at different levels. By contrast, only one SOUL gene was up-regulated by light and was highly expressed in the stigma at anthesis. Both the ELIP and SOUL genes induced by light in saffron stigmas might be associated with the structural changes affecting the chromoplast of the stigma, as a result of light exposure, which promotes the development and increases the number of plastoglobules, specialized in the recruitment of specific proteins, which enables them to act in metabolite synthesis and disposal under changing environmental conditions and developmental stages.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","format":"standard","meta":[],"pj-categs":[74],"pj-tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v19.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Identification and Cloning of Differentially Expressed SOUL and ELIP Genes in Saffron Stigmas Using a Subtractive Hybridization Approach. - Instituto Bot\u00e1nico<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/botanico.uclm.es\/en\/project\/identification-and-cloning-of-differentially-expressed-soul-and-elip-genes-in-saffron-stigmas-using-a-subtractive-hybridization-approach\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Identification and Cloning of Differentially Expressed SOUL and ELIP Genes in Saffron Stigmas Using a Subtractive Hybridization Approach. - Instituto Bot\u00e1nico\" \/>\n<meta property=\"og:url\" content=\"https:\/\/botanico.uclm.es\/en\/project\/identification-and-cloning-of-differentially-expressed-soul-and-elip-genes-in-saffron-stigmas-using-a-subtractive-hybridization-approach\/\" \/>\n<meta property=\"og:site_name\" content=\"Instituto Bot\u00e1nico\" \/>\n<meta property=\"article:modified_time\" content=\"2022-12-22T16:21:18+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/botanico.uclm.es\/en\/project\/identification-and-cloning-of-differentially-expressed-soul-and-elip-genes-in-saffron-stigmas-using-a-subtractive-hybridization-approach\/\",\"url\":\"https:\/\/botanico.uclm.es\/en\/project\/identification-and-cloning-of-differentially-expressed-soul-and-elip-genes-in-saffron-stigmas-using-a-subtractive-hybridization-approach\/\",\"name\":\"Identification and Cloning of Differentially Expressed SOUL and ELIP Genes in Saffron Stigmas Using a Subtractive Hybridization Approach. - 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Instituto Bot\u00e1nico","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/botanico.uclm.es\/en\/project\/identification-and-cloning-of-differentially-expressed-soul-and-elip-genes-in-saffron-stigmas-using-a-subtractive-hybridization-approach\/","og_locale":"en_US","og_type":"article","og_title":"Identification and Cloning of Differentially Expressed SOUL and ELIP Genes in Saffron Stigmas Using a Subtractive Hybridization Approach. - Instituto Bot\u00e1nico","og_url":"https:\/\/botanico.uclm.es\/en\/project\/identification-and-cloning-of-differentially-expressed-soul-and-elip-genes-in-saffron-stigmas-using-a-subtractive-hybridization-approach\/","og_site_name":"Instituto Bot\u00e1nico","article_modified_time":"2022-12-22T16:21:18+00:00","twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/botanico.uclm.es\/en\/project\/identification-and-cloning-of-differentially-expressed-soul-and-elip-genes-in-saffron-stigmas-using-a-subtractive-hybridization-approach\/","url":"https:\/\/botanico.uclm.es\/en\/project\/identification-and-cloning-of-differentially-expressed-soul-and-elip-genes-in-saffron-stigmas-using-a-subtractive-hybridization-approach\/","name":"Identification and Cloning of Differentially Expressed SOUL and ELIP Genes in Saffron Stigmas Using a Subtractive Hybridization Approach. - 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